Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant SARS-CoV-2 S protein RBD (K417T), His Tag (MALS verified), 100µg  

Recombinant SARS-CoV-2 S protein RBD (K417T), His Tag (MALS verified), 100µg

Recombinant SARS-CoV-2 S protein RBD (K417T), AA Arg 319 - Lys 537, expressed from human 293 cells (HEK293) His Tag (MALS verified)

Synonyms: Recombinant, Spike, S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD

More details

SPD-C52Ht-100

Availability: within 10-14 days

520,00 €

Background
It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Source
Recombinant SARS-CoV-2 S protein RBD (K417T), His Tag (SPD-C52Ht) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (K417T)).
Predicted N-terminus: Arg 319

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.5 kDa. The protein migrates as 32-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Inactivation mechanism of cold plasma combined with 222 nm ultraviolet for spike protein and its application in disinfecting of SARS-CoV-2"
Sheng, Wang, Zhao et al
J Hazard Mater (2024) 465, 133458
(2) "TXM peptides inhibit SARS-CoV-2 infection, syncytia formation, and lower inflammatory consequences"
Govednik, Lainšček, Kuhar et al
Antiviral Res (2024)
(3) "Harnessing preexisting influenza virus-specific immunity increases antibody responses against SARS-CoV-2"
Dulin, Barre, Xu et al
J Virol (2024)
Showing 1-3 of 4323 papers