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Recombinant SARS-CoV-2 S1 protein NTD (L18F, D80A, D215G, 242-244del, R246I), His Tag (MALS verified), 1mg  

Recombinant SARS-CoV-2 S1 protein NTD (L18F, D80A, D215G, 242-244del, R246I), His Tag (MALS verified), 1mg

Recombinant SARS-CoV-2 S1 protein NTD (L18F, D80A, D215G, 242-244del, R246I), known as B.1.351 or 20C/501Y.V2, AA Ser 13 - Leu 303, expressed from human 293 cells (HEK293) Fc Tag (MALS verified)

Synonyms: Recombinant, S1 protein NTD, Spike protein S1 NTD, BetaCoV S1-NTD

More details

S1D-C52Hc-1K

Availability: within 10-14 days

2 600,00 €

Background
It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Source
Recombinant SARS-CoV-2 S1 protein NTD, His Tag (S1D-C52Hc) is expressed from human 293 cells (HEK293). It contains AA Ser 13 - Leu 303 (Accession # QHD43416.1). The L18F/D80A/D215G/LAL242-244del/R246I mutations were identified on the spike protein N-terminal domain (NTD) in the SARS-CoV-2 variant (known as B.1.351 or 20C/501Y.V2) which emerged in South Africa.
Predicted N-terminus: Ser 13

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 34.5 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

References

Mapping Potential Antigenic Drift Sites (PADS) on SARS-CoV-2 Spike in Continuous Epitope-Paratope Space
Miller, Clark, Raman et al
bioRxiv (2021)

Rapidly emerging SARS-CoV-2 B.1.1.7 sub-lineage in the United States of America with spike protein D178H and membrane protein V70L mutations
Shen, Bard, Triche et al
Emerg Microbes Infect (2021)

Analysis of the molecular mechanism of SARS-CoV-2 antibodies
Jin, Wei, Sun
Biochem Biophys Res Commun (2021) 566, 45-52

Showing 1-3 of 103 papers
Please refer to product data sheet.