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Recombinant HRP-SARS-CoV-2 Spike RBD, His Tag, 200µg  

Recombinant HRP-SARS-CoV-2 Spike RBD, His Tag, 200µg

Recombinant HRP-SARS-CoV-2 Spike RBD, AA Arg 319 - Lys 537, expressed from human 293 cells (HEK293), His Tag

Synonyms: Recombinant, Spike, S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD

More details

SPD-SR2H0-200

Availability: within 10-14 days

1 380,00 €

Background
It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Source
Recombinant HRP-SARS-CoV-2 Spike RBD, His Tag (SPD-SR2H0) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1).
Predicted N-terminus: Arg 319

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.5 kDa.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by reduced SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

References

(1) "Cellular and humoral immunogenicity of a SARS-CoV-2 mRNA vaccine in patients on haemodialysis"
Strengert, Becker, Ramos et al
EBioMedicine (2021) 70, 103524
(2) "Antibody responses after a single dose of ChAdOx1 nCoV-19 vaccine in healthcare workers previously infected with SARS-CoV-2"
Havervall, Marking, Greilert-Norin et al
EBioMedicine (2021) 70, 103523
(3) "B and T cell response to SARS-CoV-2 vaccination in health care professionals with and without previous COVID-19"
Zollner, Watschinger, Rössler et al
EBioMedicine (2021) 70, 103539

Showing 1-3 of 1394 papers.
Please refer to product data sheet.