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Recombinant SARS-CoV-2 Spike NTD (C136F, Y144del, R190S, D215G, LA242-243del), His Tag (MALS verified), 1mg  

Recombinant SARS-CoV-2 Spike NTD (C136F, Y144del, R190S, D215G, LA242-243del), His Tag (MALS verified), 1mg

Recombinant SARS-CoV-2 Spike NTD (C136F, Y144del, R190S, D215G, LA242-243del), AA Ser 13 - Leu 303, expressed from human 293 cells (HEK293), His Tag (MALS verified)

Synonyms: Recombinant, S1 protein NTD, Spike protein S1 NTD, BetaCoV S1-NTD

More details

SPD-C5229-1K

Availability: within 10-14 days

2 600,00 €

Background
It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Source
Recombinant SARS-CoV-2 Spike NTD, His Tag (SPD-C5229) is expressed from human 293 cells (HEK293). It contains AA Ser 13 - Leu 303 (Accession # QHD43416.1). The mutations C136F, Y144del, R190S, D215G, LA242-243del were identified in the SARS-CoV-2 variant C.1.2.
Predicted N-terminus: Ser 13

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 34.5 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by reduced SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "A Serological Snapshot of COVID-19 Initial Stages in Israel by a 6-Plex Antigen Array"
Fisher, Levy, Fatelevich et al
Microbiol Spectr (2021)
(2) "A Combination of Receptor-Binding Domain and N-Terminal Domain Neutralizing Antibodies Limits the Generation of SARS-CoV-2 Spike Neutralization-Escape Mutants"
Haslwanter, Dieterle, Wec et al
mBio (2021)
(3) "The ongoing evolution of variants of concern and interest of SARS-CoV-2 in Brazil revealed by convergent indels in the amino (N)-terminal domain of the spike protein"
Resende, Naveca, Lins et al
Virus Evol (2021) 7 (2), veab069
Showing 1-3 of 144 papers.