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Recombinant HCoV-OC43 Spike protein, His Tag, 500µg  

Recombinant HCoV-OC43 Spike protein, His Tag, 500µg

Recombinant HCoV-OC43 Spike protein, AA Ala 14 - Pro 1297, expressed from Baculovirus-Insect cells, His Tag

Synonyms: Recombinant, Spike, S protein, Spike glycoprotein, S glycoprotein

More details

SPN-H52Hz-500

Availability: within 10-14 days

2 800,00 €

Background
Human coronavirus OC43 (HCoV-OC43) is one of seven known coronaviruses to infect humans, which is responsible for the common cold and may have been responsible for the 1889–1890 pandemic. The infecting coronavirus is an enveloped, positive-sense, single-stranded RNA virus that enters its host cell by binding to the N-acetyl-9-O-acetylneuraminic acid receptor. The spike protein of HCoV-OC43 is a trimer protruding from the viral membrane to engage cellular receptors and mediate viral fusion with host membranes. Each spike trimer contains two large regions: N-terminal S1 responsible for receptor binding and C-terminal S2 responsible for fusion.

Source
Recombinant HCoV-OC43 Spike protein, His Tag (SPN-H52Hz) is expressed from Baculovirus-Insect cells. It contains AA Ala 14 - Pro 1297 (RRSRG 754-758 GGSGG) (Accession # P36334-1).
Predicted N-terminus: Ala 14

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 148.3 kDa. The protein migrates as 150 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Peripheral lymphocyte subset counts predict antibody response after SARS-CoV-2 mRNA-BNT162b2 vaccine in cancer patients: Results from the Vax-On-Profile study"
Nelli, Fabbri, Panichi et al
Int Immunopharmacol (2022) 108, 108774
(2) "Increased resistance of SARS-CoV-2 Lambda variant to antibody neutralization"
Guo, Fan, Song et al
J Clin Virol (2022) 150-151, 105162
(3) "Isolation, characterization, and structure-based engineering of a neutralizing nanobody against SARS-CoV-2"
Li, Zhou, Li et al
Int J Biol Macromol (2022)
Showing 1-3 of 20728 papers.