Recombinant human CNTN2 / Contactin-2 Protein, 100µg

Background
Contactin-2 also known as CNTN2, TAX1 (transiently-expressed axonal glycoprotein), TAG1 (transient axonal glycoprotein), and axonin-1, and is a member of the immunoglobulin superfamily. CNTN2 consists of six Ig-like domains and four fibronectin type III domains, and is anchored to the membrane by glycosylphosphatidylinositol (GPI), whereas the soluble form can be released by a GPI-specific phospholipase. As a neural cell adhesion molecule expressed by a subset of neuronal populations in the developing CNS and PNS, CNTN2 mediates cell-cell interactions either via homophilic, or heterophilic contacts with various adhesion molecules including NgCAM, NrCAM, NCAM and neurocan. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. It may also be involved in glial tumorigenesis and may provide a potential target for therapeutic intervention.

Source
Recombinant Human Contactin-2 /CNTN2 Protein, His Tag (CN2-H5226) is expressed from human 293 cells (HEK293). It contains AA Ser 31 - Asn 1012 (Accession # NP_005067.1).
Predicted N-terminus: Ser 31

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 108.3 kDa. The protein migrates as 116-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
N/A

Clinical and Translational Updates

(1) "Proteomic signatures of physical, cognitive, and imaging outcomes in multiple sclerosis"
Jalaleddini, Jakimovski, Keshavan et al
Ann Clin Transl Neurol (2024)
(2) "Association between serum multi-protein biomarker profile and real-world disability in multiple sclerosis"
Zhu, Chen, Zhang et al
Brain Commun (2024) 6 (1), fcad300
(3) "Interplay between intrinsic flexibility and sugar coating in Contactin-2 homodimerization"
Sen
Structure (2024) 32 (1), 3-5
Showing 1-3 of 319 papers.