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Recombinant Human CD30 / TNFRSF8 Protein, Llama IgG2b Fc Tag, low endotoxin, 100 µg  

Recombinant Human CD30 / TNFRSF8 Protein, Llama IgG2b Fc Tag, low endotoxin, 100 µg

Recombinant human CD30, Phe 19 - Lys 379, expressed from human 293 cells (HEK293), Llama IgG2b Fc Tag, low endotoxin

Synonym
recombinant, human, protein, TNFRSF8, CD30, D1S166E, Ki-1

More details

TN8-H5250-100

420,00 €

Background
Human CD30 is also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. TNFRSF-8 is expressed by activated, but not by resting, T and B cells. Also, CD30 is expressed on classical Hodgkin Lymphoma cells together with CD15. CD30 is the receptor for TNFSF8/CD30L. CD30 can interact with TRAF2 and TRAF5, and mediate the signal transduction that leads to the activation of NF-kappa-B. TNFRSF8 may play a role in the regulation of cellular growth and transformation of activated lymphoblasts. TNFRSF8 is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity.

Source
Recombinant human CD30, Llama IgG2b Fc Tag, low endotoxin (TN8-H5250) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Lys 379 (Accession # NP_001234.2).
Predicted N-terminus: Phe 19

Molecular Characterization
This protein carries a llama IgG2b Fc tag at the C-terminus.
The protein has a calculated MW of 66.3 kDa. The protein migrates as 95-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 0.01 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Human CD30, Llama IgG2b Fc Tag, low endotoxin (Cat. No. TN8-H5250) at 0.5 μg/mL (100 μL/well) can bind Anti-CD30 Mab, Human IgG1 with a linear range of 0.1-1.6 ng/mL (Routinely tested).
Measured by its binding ability in a functional ELISA. Immobilized Human CD30 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CDL-H525b) at 0.5 μg/mL (100 μL/well) can bind Human CD30, Llama IgG2b Fc Tag, low endotoxin (Cat. No. TN8-H5250) with a linear range of 10-78 ng/mL (QC tested).

References

(1) Gorczyca W., et al., 2003, Int. J. Oncol. 22 (2): 319–24.
(2) Aizawa S., et al., 1997, J. Biol. Chem. 272 (4): 2042–5.
(3) Ansieau S., 1996, Proc. Natl. Acad. Sci. U.S.A. 93 (24): 14053–8.
(4) Lee S.Y., et al., 1996, J. Exp. Med. 183:669-674.