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Recombinant Human LAG-3 / CD223 Protein, Llama IgG2b Fc Tag, low endotoxin, 100 µg  

Recombinant Human LAG-3 / CD223 Protein, Llama IgG2b Fc Tag, low endotoxin, 100 µg

Recombinant human LAG-3, Leu 23 - Leu 450, expressed from human 293 cells, Llama IgG2b Fc Tag

Synonym
recombinant, human, protein, LAG3, CD223, FDC

More details

LA3-H525c-100

420,00 €

Background
Lymphocyte activation gene 3 protein (LAG3) is also known as CD antigen CD223 and protein FDC, which belongs to immunoglobulin (Ig) superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. LAG3 /CD223 involved in lymphocyte activation. LAG3 /CD223 binds to HLA class-II antigens.

Source
Recombinant Human LAG-3, Llama IgG2b Fc Tag, low endotoxin (LA3-H525c) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Leu 450 (Accession # P18627-1). 
Predicted N-terminus: Leu 23

Molecular Characterization
This protein carries a llama IgG2b Fc tag at the C-terminus.
The protein has a calculated MW of 74.0 kDa. The protein migrates as 90 KDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 0.01 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arg, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
4-8°C for 12 months in lyophilized state;
-80°C for 6 months under sterile conditions after reconstitution.

Bioactivity-ELISA
Immobilized Human LAG-3, Llama IgG2b Fc Tag, low endotoxin (Cat. No. LA3-H525c) at 2 μg/mL (100 μL/well) can bind Anti-Human LAG3 MAb with a linear range of 0.2-4 ng/mL (QC tested).Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag.

References

(1) Triebel F., et al., 1990, J. Exp. Med. 171:1393-1405.
(2) Baixeras E., et al., 1992, J. Exp. Med. 176:327-337.

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