Recombinant Human LRP-6 (20-630) Protein, Mouse IgG2a Fc Tag, 100 µg

Background
LRP6 is a transmembrane low-density lipoprotein receptor that shares a similar structure with LRP5. Low-density lipoprotein receptor-related protein 6 is a key component of the LRP5/LRP6/Frizzled co-receptor group that is involved in canonical Wnt pathway. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin.

Source
Recombinant Human LRP-6 (20-630), Mouse IgG2a Fc Tag (LR6-H5253) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Pro 630 (Accession # O75581-1 ).
Predicted N-terminus: Ala 20

Molecular Characterization
This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 95.8 kDa. The protein migrates as 55 kDa and 110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 0.1 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "MiR-539-3p impairs osteogenesis by suppressing Wnt interaction with LRP-6 co-receptor and subsequent inhibition of Akap-3 signaling pathway
Tripathi, John, Kumar et al
Front Endocrinol (Lausanne) (2022) 13, 977347
(2) "The Low-density Lipoprotein Receptor-related Protein 6 Pathway in the Treatment of Intestinal Barrier Dysfunction Induced by Hypoxia and Intestinal Microbiota through the Wnt/β-catenin Pathway
Liu, Li, Liu et al
Int J Biol Sci (2022) 18 (11), 4469-4481
(3) "Combined Transcriptomic and Protein Array Cytokine Profiling of Human Stem Cells from Dental Apical Papilla Modulated by Oral Bacteria
Zymovets, Razghonova, Rakhimova et al
Int J Mol Sci (2022) 23 (9)
Showing 1-3 of 47 papers.