Recombinant Human Decorin Protein, His Tag, 200 µg

Background
Decorin is also known as bone proteoglycan II, PGS2, SLRR1B, DCN, DSPG2 and PG40, is a secreted chondroitin /dermatan sulfate proteoglycan in the family of small leucine-rich proteoglycans (SLRPs). Decorin is a small cellular or pericellular matrix proteoglycan and is closely related in structure to biglycan protein. Decorin and biglycan are thought to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. Decorin appears to influence fibrillogenesis, and also interacts with fibronectin, thrombospondin, the complement component C1q, epidermal growth factor receptor (EGFR) and transforming growth factor-beta (TGF-beta). Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD).

Source
Recombinant Human Decorin, His Tag (DE1-H5223) is expressed from human 293 cells (HEK293). It contains AA Gly 17 - Lys 359 (Accession # NP_001911.1).
Predicted N-terminus: Gly 17

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 38.8 kDa. The protein migrates as 46-55 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20^°C to -70^°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Collagen molecular organization preservation in human fascia lata and periosteum after tissue engineering"
Vettese, Manon, Chretien et al
Front Bioeng Biotechnol (2024) 12, 1275709
(2) "Myokines and Biomarkers of Frailty in Older Inpatients with Undernutrition: A Prospective Study"
Liu, Li, Zhu et al
J Frailty Aging (2024) 13 (2), 82-90
(3) "Exercise training mode effects on myokine expression in healthy adults: A systematic review with meta-analysis"
Bettariga, Taaffe, Galvão et al
J Sport Health Sci (2024)
Showing 1-3 of 3264 papers.