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Recombinant SARS-CoV-2 (COVID-19) Nucleocapsid protein, His Tag, 100µg  

Recombinant SARS-CoV-2 (COVID-19) Nucleocapsid protein, His Tag, 100µg

Recombinant SARS-CoV-2 (COVID-19) Nucleocapsid protein, AA Met 1 - Ala 419, expressed from human 293 cells (HEK293), His Tag

Synonyms: Recombinant, Nucleocapsid protein, NP, Protein N, COVID-19

More details

NUN-C5227-100

Availability: within 7 days

520,00 €

Background
Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.

Source
Recombinant SARS-CoV-2 Nucleocapsid protein, His Tag (NUN-C5227) is expressed and secreted by human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1).
Predicted N-terminus: Met 1

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 47.3 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Bioactivity
Please refer to product data sheet.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Comparison of two T cell assays to evaluate T cell responses to SARS-CoV-2 following vaccination in naïve and convalescent healthcare workers"
Phillips, Adele, Malone et al
Clin Exp Immunol (2022)
(2) "Multiplexed COVID-19 antibody quantification from human sera using label-free nanoplasmonic biosensors"
Adi, Biswas, Shelef et al
Biomed Opt Express (2022) 13 (4), 2130-2143
(3) "Targeting the N-Terminus Domain of the Coronavirus Nucleocapsid Protein Induces Abnormal Oligomerization via Allosteric Modulation"
Hsu, Chen, Lin et al
Front Mol Biosci (2022) 9, 871499
Showing 1-3 of 52462 papers.

Citations

(1) "Clinical validation of an open-access SARS-COV-2 antigen detection lateral flow assay, compared to commercially available assays"
Authors: Bachman CM, Grant BD, Anderson CE, et al.
Journal: PloS one 2021
Application: Binding Assay
(2) "Variable post-translational modifications of SARS-CoV-2 nucleocapsid protein"
Authors: Supekar NT, Shajahan A, Gleinich AS, et al.
Journal: Glycobiology 2021
Application: MALDI-MS
(3) "Orthogonal immunoassays for IgG antibodies to SARS-CoV-2 antigens reveal that immune response lasts beyond 4 mo post illness onset"
Authors: Sasisekharan, Varun et al.
Journal: Proc Natl Acad Sci U S A 2021
Application: ELISA
(4) "SARS-CoV-2 exposure, symptoms and seroprevalence in healthcare workers in Sweden"
Authors: Rudberg AS, Havervall S, Månberg A, et al.
Journal: Nature communications 2020
Application: Luminex

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