Recombinant SARS-CoV-2 (COVID-19) S2 protein, His Tag, 100µg

Background
It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Source
Recombinant SARS-CoV-2 S2 protein, His Tag (S2N-C52H5) is expressed from human 293 cells (HEK293). It contains AA Ser 686 - Pro 1213 (Accession # QHD43416.1). Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) are introduced to prevent the formation of aggregates in the course of protein production.

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 60.0 kDa. The protein migrates as 65-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Bioactivity
Please refer to product data sheet.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1)"SARS-CoV-2 spike N-terminal domain modulates TMPRSS2-dependent viral entry and fusogenicity"
Meng, Datir, Choi et al
Cell Rep (2022)
(2) "The prospective screening for SARS-CoV-2 S1/S2 antibodies delineates the factual incidence of COVID-19 and shows a sustained serological response post COVID-19 in kidney transplant recipients"
Firket, Bouquegneau, Seidel et al
Acta Clin Belg (2022)
(3) "The SARS-CoV-2 Nucleoprotein Induces Innate Memory in Human Monocytes"
Urbán, Italiani, Boraschi et al
Front Immunol (2022) 13, 963627
Showing 1-3 of 883 papers.

Citations
(1) "HDL-scavenger receptor B type 1 facilitates SARS-CoV-2 entry"
Authors: Wei C, Wan L, Yan Q, et al.
Journal: Nature metabolism 2020
Application: SPR & FACS & Confocal microscopy
(2) "Immune responses to SARS-CoV-2 in three children of parents with symptomatic COVID-19"
Authors: Tosif S, Neeland MR, Sutton P, et al.
Journal: Nature communications 2020
Application: ELISA & FACS