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Background
Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
Source
Recombinant Biotinylated SARS-CoV-2 Nucleocapsid protein, His,Avitag (NUN-C81Q6) is expressed from E.coli cells. It contains AA Met 1 - Ala 419 (Accession # QHO62115.1).
Predicted N-terminus: Met
Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 51.1 kDa. The protein migrates as 52 kDa under reducing (R) condition (SDS-PAGE).
Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Passed as determined by the HABA assay / binding ELISA.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, Arginine, pH7.5. Normally trehalose is added as protectant before lyophilization.
Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.
Bioactivity
Please refer to product data sheet.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
(1) "Preparation and pre-clinical evaluation of flagellin-adjuvanted NOM vaccine candidate formulated with Spike protein against SARS-CoV-2 in mouse model"
Farshidi, Farshidi, Ghaedi et al
Microb Pathog (2022)
(2) "Age- and sex-based changes in spike protein antibody status after SARS-CoV-2 vaccination and effect of past-infection in healthcare workers in Osaka"
Hoshida, Koeda, Hattori et al
BMC Infect Dis (2022) 22 (1), 709
(3) "Exosomes from WSSV-infected shrimp contain viral components that mediate virus infection"
Sun, Zhang
J Gen Virol (2022) 103 (8)
Showing 1-3 of 52963 papers.
Recombinant SARS-CoV-2 (COVID-19) S1 protein, His Tag (MALS verified), 100µg - 494,00 €
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Recombinant Biotinylated SARS-CoV-2 (COVID-19) S1 protein, His,Avitag™ (MALS verified), 25µg - 494,00 €
Recombinant SARS-CoV-2 (COVID-19) Nucleocapsid protein, His Tag, 100µg - 520,00 €
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