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Background
Human coronavirus NL63 (HCoV-NL63) is one of seven known coronaviruses to infect humans. The virus is an enveloped, positive-sense, single-stranded RNA virus which enters its host cell by binding to ACE2. Infection with the virus has been confirmed worldwide, and has an association with many common symptoms and diseases. The spike protein of HCoV-NL63 is a trimer protruding from the viral membrane to engage cellular receptors and mediate viral fusion with host membranes. Each spike trimer contains two large regions: N-terminal S1 responsible for receptor binding and C-terminal S2 responsible for fusion.
Source
Recombinant HCoV-NL63 S1 protein, His Tag (SIN-V52H3) is expressed from human 293 cells (HEK293). It contains AA Cys 19 - Val 717 (Accession # Q6Q1S2-1).
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 79.5 kDa. The protein migrates as 120-140 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization.
Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.
Bioactivity
Please refer to product data sheet.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
(1) "Triterpene Derivatives as Potential Inhibitors of the RBD Spike Protein from SARS-CoV-2: An In Silico Approach"
Avelar, Pedraza-González, Sinicropi et al
Molecules (2023) 28 (5)
(2) "Folic Acid and Leucovorin Have Potential to Prevent SARS-CoV-2-Virus Internalization by Interacting with S-Glycoprotein/Neuropilin-1 Receptor Complex"
Škrbić, Travar, Stojiljković et al
Molecules (2023) 28 (5)
(3) "Comparative humoral response of mRNA and inactivated vaccines against COVID-19 in healthy adults aged 60 years and older"
Güvenir, Yürüker, Yetkin et al
J Infect Dev Ctries (2023) 17 (2), 178-181
Showing 1-3 of 2590 papers.
Recombinant SARS-CoV-2 (COVID-19) S1 protein, His Tag (MALS verified), 100µg - 494,00 €
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Recombinant Biotinylated SARS-CoV-2 (COVID-19) S1 protein, His,Avitag™ (MALS verified), 25µg - 494,00 €
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