Recombinant HCoV-HKU1(isolate N5) S1 protein, His Tag, 100µg

Background
HCoV-HKU1, formally known as Human Coronavirus Hong Kong University 1, is a member of the Betacoronavirus genus within the Coronaviridae family. It was first identified in Hong Kong in 2005 and has since been detected in various parts of the world, causing respiratory infections ranging from asymptomatic to severe cases.
HCoV-HKU1 is an enveloped virus, meaning its genetic material is contained within a lipid membrane surrounded by structural proteins. This envelope is studded with glycoproteins, including the Spike Protein (S Protein), which plays a critical role in virus attachment and entry into host cells. The S Protein is a type I transmembrane protein that protrudes from the virus surface, serving as a bridge between the virus and its host cell receptor. Once the S Protein binds to its cognate receptor on the host cell surface, it triggers a series of events leading to the fusion of the viral and cellular membranes, allowing the release of the virus's genetic material into the host cell cytoplasm.

Source
Recombinant HCoV-HKU1(isolate N5) S1 protein, His Tag (SIN-V52H6) is expressed from human 293 cells (HEK293). It contains AA Ala 13 - Arg 756 (Accession # Q0ZME7-1).
Predicted N-terminus: Ala 13

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 85.7 kDa. The protein migrates as 110-130 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. .

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Bioactivity
Please refer to product data sheet.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Investigation of IgG Titers in Hemodialysis Patients and Controls Following Administration of the COVID-19 Vaccine"
Jozpanahi, Jafari, Moharrer et al
Int Tinnitus J (2024) 27 (2), 183-190
(2) "Functional dissection of the spike glycoprotein S1 subunit and identification of cellular cofactors for regulation of swine acute diarrhea syndrome coronavirus entry"
Yang, Wang, Li et al
J Virol (2024)
(3) "Characteristics of neutralizing antibody titers of the SARS-CoV-2 vaccine in maintenance hemodialysis patients"
Amari, Morimoto, Teranishi et al
Clin Nephrol (2024)
Showing 1-3 of 2946 papers.