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Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM110) (Delta Specific), 100µg  

Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM110) (Delta Specific), 100µg

Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM110) (Delta Specific, B.1.617.2/AY.4)

Isotype: Human IgG1/kappa

More details

SPD-M370-100

Availability: within 7 days

869,00 €

Background
It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Source
Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Delta Specific) is a chimeric monoclonal antibody recombinantly expressed from HEK293 cells, which combines the variable region of a mouse monoclonal antibody with human IgG1 constant domain. The mouse monoclonal antibody was obtained from a mouse immunized with recombinant SARS-CoV-2 Spike RBD protein. The antibody is specific against the Delta (B.1.617.2/AY.4) variant of SARS-CoV-2, and has no binding with the spike RBD of the wild type virus and the Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1), Omicron (B.1.1.529/BA.1) variant.

Isotype
Human IgG1/kappa

Specificity
This product is a specific antibody against SARS-CoV-2 Spike protein RBD domain. Cross-reactivity with Spike protein RBD domain of other coronaviruses, including SARS-CoV, MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1, has not been tested.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Bioactivity
Please refer to product data sheet.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

Olearo, Radmanesh, Felber et al. (2022) "Anti-SARS-CoV-2 antibodies in breast milk during lactation after infection or vaccination: A cohort study" J Reprod Immunol 153, 103685

Okada, Ikeda-Ishizaka, Ono et al. (2022) "Establishment of Neutralizing Monoclonal Antibodies Against Severe Acute Respiratory Syndrome Coronavirus 2 by the Screening with Exosomes Expressing the Viral Spike Protein" , Monoclon Antib Immunodiagn Immunother 41 (4), 173-180

Meira, Silva, Santos et al. (2022) "Diagnostic Performance of Three ELISAs for Detection of Antibodies against SARS-CoV-2 in Human Samples" ScientificWorldJournal , 7754329

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