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Recombinant Mouse ErbB2/HER2 Protein, His Tag, 100µg  

Recombinant Mouse ErbB2/HER2 Protein, His Tag, 100µg

Recombinant Mouse HER2 /ErbB2 /CD340 Protein (mouse HER2) Thr23-Thr653 produced in human HEK293 cells, His Tag

Synonym
recombinant mouse protein ERBB2, CD340, HER-2/neu, HER2 protein

More details

ER2-M5220-100

Availability: within 7 days

455,00 €

Background
Human Epidermal growth factor Receptor 2 (HER2) is also called ERBB2, HER-2,HER-2 /neu, NEU, NGL,TKR1 and c-erb B2,and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30% of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation .

Source
Recombinant Mouse HER2 /ErbB2 /CD340 Protein, His Tag (ER2-M5220) is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 653 (Accession # AAH53078.1).
Predicted N-terminus: Thr 23

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 70.6 kDa. The protein migrates as 80-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Resistance to CDK4/6 inhibition: Mechanisms and strategies to overcome a therapeutic problem in the treatment of hormone receptor-positive metastatic breast cancer"
Papadimitriou, Pazaiti, Iliakopoulos et al
Biochim Biophys Acta Mol Cell Res (2022)
(2) "HER2-positive/ER-low breast carcinoma shows a response to neoadjuvant chemotherapy similar to that of HER2-positive/ER-negative breast carcinoma"
Weisman, Ospina-Romero, Yu et al
Pathol Res Pract (2022) 238, 154087
(3) "Margetuximab with retifanlimab as first-line therapy in HER2+/PD-L1+ unresectable or metastatic gastroesophageal adenocarcinoma: MAHOGANY cohort A"
Catenacci, Kang, Yoon et al
ESMO Open (2022) 7 (5), 100563
Showing 1-3 of 32662 papers.