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Recombinant human DKK-3 /DKK3 Protein, 200µg  

Recombinant human DKK-3 /DKK3 Protein, 200µg

Recombinant human DKK-3 /DKK3 Protein (rhDKK3) (R335G) AA Ala 22 - Ile 350 expressed in human HEK293 cells, His Tag

Synonym: recombinant, human, protein DKK3, DKK-3, REIC, RIG, Dickkopf-related protein 3

More details

DK3-H5220-200

Availability: within 7 days

468,00 €

Background
Members of the dickkopf-related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. DKK3 has been proposed as tumour suppressor gene and a marker for tumour blood vessels(1). DKK3 is the only DKK family member abundantly expressed in normal lung, but silenced by promoter hypermethylation in a large fraction of lung cancer cell lines and lung tumors. Downregulation of DKK3 was correlated with tumor progression and expression of nuclear beta-catenin in lung tumors. Ectopic expression of DKK3 in lung cancer cells with DKK3 hypermethylation induced apoptosis and inhibited TCF-4 activity as well as nuclear accumulation of beta-catenin and expression of TCF-4 targets c-Myc and cyclin D1.(2) DKK3 modulates FGF and Activin/Nodal signaling to regulate mesoderm induction during early Xenopus development, was reported(3).

Source
Recombinant Human Dkk-3 (R335G), His Tag (DK3-H5220) is expressed from human 293 cells (HEK293). It contains AA Ala 22 - Ile 350 (Accession # Q9UBP4-1(R335G)).
Predicted N-terminus: Ala 22

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 37.0 kDa. The protein migrates as 45-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhDKK3 by the LAL method.

Purity
>90% as determined by SDS-PAGE of reduced rhDKK3.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
N/A

Clinical and Translational Updates

(1) "The role of Wnt pathway antagonists in early-stage lung adenocarcinoma"
Zeybek, Öz, Kalemci et al
Mol Biol Rep (2021)
(2) "REIC/Dkk-3 Gene Therapy Induces Immunogenic Cell Death in a Mouse Model of Malignant Mesothelioma"
Araki, Yamamuro, Tomonobu et al
Anticancer Res (2021) 41 (10), 4837-4855
(3) "Association of Urinary Dickkopf-3 with Residual Renal Function Decline in Patients Undergoing Peritoneal Dialysis"
Torigoe, Muta, Tsuji et al
Medicina (Kaunas) (2021) 57 (6)
Showing 1-3 of 173 papers.