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Recombinant human DKK-3 /DKK3 Protein, 20µg  

Recombinant human DKK-3 /DKK3 Protein, 20µg

Recombinant human DKK-3 /DKK3 Protein (rhDKK3) Pro23-Ile350 expressed in human HEK293 cells

Synonym: recombinant, human, protein DKK3, DKK-3, REIC, RIG, Dickkopf-related protein 3

More details

DK3-H5220-20

Availability: within 7 days

144,00 €

Background
Members of the dickkopf-related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. DKK3 has been proposed as tumour suppressor gene and a marker for tumour blood vessels(1). DKK3 is the only DKK family member abundantly expressed in normal lung, but silenced by promoter hypermethylation in a large fraction of lung cancer cell lines and lung tumors. Downregulation of DKK3 was correlated with tumor progression and expression of nuclear beta-catenin in lung tumors. Ectopic expression of DKK3 in lung cancer cells with DKK3 hypermethylation induced apoptosis and inhibited TCF-4 activity as well as nuclear accumulation of beta-catenin and expression of TCF-4 targets c-Myc and cyclin D1.(2) DKK3 modulates FGF and Activin/Nodal signaling to regulate mesoderm induction during early Xenopus development, was reported(3).

Source
Recombinant human DKK-3 /DKK3 Protein (rhDKK3) Pro23-Ile350 (Accession # NP_001018067.1) was expressed in human cells HEK293 at ACRObiosystems.

Molecular Characterization
rhDKK3, a 340 amino acids protein with polyhistidine tag at the C-terminus, and has a calculated MW of 37.6 kDa. The predicted N-terminus is Pro23. DTT-reduced protein migrates as 50-60 kDa protein due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhDKK3 by the LAL method.

Purity
>95% as determined by SDS-PAGE of reduced rhDKK3.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4C-8C); After reconstitution under sterile conditions for 1 month (4C-8C) or 3 months (-20C to -70C).

Bioactivity
N/A

References

(1)Medinger M, Tzankov A,et al.( 2011), Thromb Haemost. 105(1):72-80
(2)Yue W, Sun Q, et al.(2008). Carcinogenesis. 29(1):84-92.
(3)Pinho S, Niehrs C.et al.(2007). Differentiation. 75(10):957-67.