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Recombinant human PCSK9 Protein, His Tag, 50µg  

Recombinant human PCSK9 Protein, His Tag, 50µg

Recombinant human PCSK9(rhPCSK9) Gln31-Gln692, produced in human HEK293 cells, His Tag

recombinant human protein, PCSK9, FH3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, Proprotein convertase subtilisin/kexin type 9

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420,00 €

Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene.This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons.(1) Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).(2,3).

Recombinant Human PCSK9, His Tag (PC9-H5223) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 692 (Accession # AAI66619). Predicted N-terminus: Gln 31

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 75.1 kDa. The protein migrates as 20 kDa and 65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation and proteolytic digestion.

Less than 1.0 EU per 1 μg of the rhPCSK9 by the LAL method.

>97% as determined by SDS-PAGE. All lots are greater than 97% pure.

Bulk protein in a 0.22 μm filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder.Normally trehalose and mannitol are added as protectants before lyophilization.

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Immobilized Human LDL R, His Tag (Cat. No. LDR-H5224) at 10 μg/mL (100 μL/well) can bind Human PCSK9, His Tag (Cat. No. PC9-H5223) with a linear range of 0.031-0.125 μg/mL (QC tested).
FACS analysis shows that Human PCSK9, His Tag (Cat. No. PC9-H5223) inhibits LDL uptake in HepG2 cells. The EC50 for this effect is 0.2857-0.4668 μg/mL.


(1) Seidah NG, et al., Proc. Natl. Acad. Sci. U.S.A. 100 (3): 928–33.
(2) Abifadel, M. et al., 2003, Nat. Genet. 34: 154-156.
(3) Dubuc G. et al., 2004, Arterioscler. Thromb. Vasc. Biol. 24 (8): 1454–9.