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Recombinant human ApoA1 Protein, 50µg  

Recombinant human ApoA1 Protein, 50µg

Recombinant Human Apo-A1 Protein (Human Apo-A1, His Tag) Arg 19 - Gln 267 was produced in human 293 cells (HEK293)

Synonym: recombinant, human, protein, Apolipoprotein A-I, APOA1, MGC117399

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Availability: within 7 days

384,00 €

ApoA1 is also known as apolipoprotein A-I, ApoA-I , and is the major protein component of high density lipoprotein (HDL) in plasma. It has a specific role in lipid metabolism. Chylomicrons secreted from the intestinal enterocyte also contain ApoA1 but it is quickly transferred to HDL in the bloodstream [1]. The protein promotes cholesterol efflux from tissues to the liver for excretion. It is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. ApoA-I was also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect.[2] Defects in the gene encoding it are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis.[3] In addition, it has been shown that ApoA1 is implicated in the anti-endotoxin function of HDL via interaction with lipopolysaccharide or endotoxin. [4]

Recombinant Human Apo-A1 Protein (Human Apo-A1, His Tag), His Tag (AP1-H5225) is expressed from human 293 cells (HEK293). It contains AA Arg 19 - Gln 267 (Accession # NP_000030.1).
Predicted N-terminus: Arg 19

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 29.8 kDa. The protein migrates as 27 kDa under reducing (R) condition (SDS-PAGE).

Less than 1.0 EU per μg of the rhApoA1 by the LAL method.

>95% as determined by SDS-PAGE.

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).



(1) Wasan KM , et al. 2008, Nature Reviews Drug Discovery 7 (1): 84–99.
(2) Yui Y, et al. 1988, J. Clin. Invest. 82 (3): 803–7.
(3) Eggerman, T.L. et al., 1991, J. Lipid Res. 32: 821-828.
(4) Voyiaziakis, E. et al., 1998. J. Lipid Res. 39: 313-321.