Recombinant human EPHB4 Protein, 200µg

Background
Ephrin type-B receptor 4(EPHB4) is also known as HTK, MYK1 and TYRO11,is a member of Eph family. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by EPHB4 binds to Ephrin-B2 and plays an essential role in vascular development. (1) EPHB4 and its ligand ephrin-B2 are specifically expressed on venous and arterial endothelial cells, respectively, and play an essential role in vascular development via bidirectional signals. The forward EPHB4 signaling inhibits cell adhesion, chemotaxis, angiogenesis and tumor growth. Incontrast, the reverse Ephrin-B2 signaling exerts the opposite effect. It has been reported that aberrant expression of EPHB4 is associated with prostate cancer and highly malignant breast cancers, accordingly, EPHB4 has potential application as a therapeutic candidate.

Source
Recombinant Human EPH Receptor B4 / EPHB4 Protein (rhEPHB4), His Tag (EP4-H5229) is expressed from human 293 cells (HEK293). It contains AA Leu 16 - Ala 539 (Accession # NP_004435.3).
Predicted N-terminus: Leu 16

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 57.9 kDa. The protein migrates as 65-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>97% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20^°C to -70^°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Genetic testing in the evaluation of individuals with clinical diagnosis of atypical Sturge-Weber syndrome"
Yeom, Cohen, Weiss et al
Am J Med Genet A (2023)
(2) "Fetal hydrothorax treated with pleuro-amniotic shunting: fetal and maternal complication and long-term outcomes"
Lanna, Consonni, Faiola et al
Fetal Diagn Ther (2023)
(3) "Bone circuitry and interorgan skeletal crosstalk"
Zaidi, Kim, Mathew et al
Elife (2023) 12
Showing 1-3 of 812 papers.