Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant human EPHB4 Protein, 200µg  

Recombinant human EPHB4 Protein, 200µg

Recombinant Human EPH Receptor B4 / EPHB4 Protein (rhEPHB4) Glu17-Ala539 was produced in human 293 cells (HEK293)

Synonym
recombinant human protein EphB4, HTK, MYK1, TYRO11, Ephrin-type-B-receptor-4

More details

EP4-H5229-200

Availability: within 7 days

348,00 €

Background
Ephrin type-B receptor 4(EPHB4) is also known as HTK, MYK1 and TYRO11,is a member of Eph family. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by EPHB4 binds to Ephrin-B2 and plays an essential role in vascular development. (1) EPHB4 and its ligand ephrin-B2 are specifically expressed on venous and arterial endothelial cells, respectively, and play an essential role in vascular development via bidirectional signals. The forward EPHB4 signaling inhibits cell adhesion, chemotaxis, angiogenesis and tumor growth. Incontrast, the reverse Ephrin-B2 signaling exerts the opposite effect. It has been reported that aberrant expression of EPHB4 is associated with prostate cancer and highly malignant breast cancers, accordingly, EPHB4 has potential application as a therapeutic candidate.

Source
Recombinant Human EPH Receptor B4 / EPHB4 Protein (rhEPHB4) Glu17-Ala539 (NP_004435.3) was produced in human 293 cells (HEK293)

Molecular Characterization
rhEPHB4 contains C-terminal polyhistidine tag and has a calculated MW of 57.8 kDa. As a result of glycosylation, DTT-reduced protein migrates as 65 - 70 kDa polypeptide in SDS-PAGE.

Endotoxin
Less than 1.0 EU per μg of the rhEPHB4 by the LAL method.

Purity
>97% as determined by SDS-PAGE.

Formulation
Refer to data sheet

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized rhEPHB4 at 2 μg/ml ( 100 μl/well ) can bind human EphrinB2 with a linear range of 0.8 - 85 ng/ml.

References

(1) Bennett, B.D. et al., 1994, J. Biol. Chem. 269: 14211-14218.
(2) Noren, N.K. et al., 2006, Nat. Cell. Biol. 8: 815-825.
(3) Xia, G. et al., 2005, Cancer Res. 65: 4623-4632.
(4) Kertesz, N. et al., 2006, Blood. 107: 2330-2338.