Recombinant human NTRK2 /TRKB Protein, Fc Tag, 100µg

Background
Neurotrophic tyrosine kinase receptor type 2 (NTRK2) also known as BDNF/NT-3 growth factors receptor, Tropomyosin-related kinase B (TRKB) and TrkB tyrosine kinase, which belongs to the protein kinase superfamily or Tyr protein kinase family. Insulin receptor subfamily. NTRK2 / TrkB contains two Ig-like C2-type (immunoglobulin-like) domains, two LRR (leucine-rich) repeats, one LRRCT domain, one LRRNT domain, one protein kinase domain. NTRK2 / Trk-B is expressed in the central and peripheral nervous system. The catalytic activity of NTRK2 is “ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate”. NTRK2 / TrkB involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity.

Source
Recombinant Human NTRK2 /TRKB Protein, Fc Tag (NT2-H5254) is expressed from human 293 cells (HEK293). It contains AA Cys 32 - His 430 (Accession # AAH31835).
Predicted N-terminus: Cys 32

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 70.4 kDa. The protein migrates as 86-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>98% purity as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20^°C to -70^°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
N/A

Clinical and Translational Updates

(1) Allen S.J., et al., 1994, Neuroscience 60:825-834.
(2) Stoilov P., et al., 2002, Biochem. Biophys. Res. Commun. 290:1054-1065.
(3) Meakin S.O., et al., 1999, J. Biol. Chem. 274:9861-9870.
(4) Yeo G.S., et al., 2004, Nat. Neurosci. 7:1187-1189.

Citations

(1) "SIPA1L2 controls trafficking and signaling of TrkB-containing amphisomes at presynaptic terminals"
Authors: M Andres-Alonso, et al
Journal: BioRxiv  43516
Application: Cell Culture