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Recombinant Human BMPR-1A / ALK-3 Protein, Fc Tag, 200µg  

Recombinant Human BMPR-1A / ALK-3 Protein, Fc Tag, 200µg

Recombinant Human Bone morphogenetic protein receptor (BMPR)-1A / ALK-3 Protein, AA Gln 24 - Arg 152, expressed from human 293 cells (HEK293), Fc Tag

Synonym: recombinant, human, protein BMPR-1A, ALK-3, ACVRLK3, CD292, SKR5, bone morphogenetic protein receptor type IA

More details

AL3-H5259-200

Availability: within 7 days

288,00 €

Background
The bone morphogenetic protein receptor, type IA also known as BMPR1A or ALK3 is a protein which in humans is encoded by the BMPR1A gene. BMPR1A has also been designated as CD292 (cluster of differentiation 290). The bone morphogenetic protein (BMP) receptors are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR1A (this protein) and BMPR1B and the type II receptor BMPR2. These receptors are also closely related to the activin receptors, ACVR1 and ACVR2[1]. Alk3-mediated BMP signaling in AV endocardial/mesenchymal cells plays a central role during cushion morphogenesis[2].

Source
Recombinant, human Bone morphogenetic protein receptor,type IA Fc Chimera (rhBMPR1A-Fc or rhALK3-Fc), Fc Tag (AL3-H5259) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Arg 152 (Accession # P36894-1).
Predicted N-terminus: Gln 24

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 40.3 kDa. The protein migrates as 56 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhALK3-Fc by the LAL method.

Purity
>98% purity as determined by SDS-PAGE of reduced rhALK3-Fc.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "BMPR1A is necessary for chondrogenesis and osteogenesis, whereas BMPR1B prevents hypertrophic differentiation"
Mang, Kleinschmidt-Doerr, Ploeger et al
J Cell Sci (2020) 133 (16)
(2) "Transforming growth factor-beta family members are regulated during induced luteolysis in cattle"
Haas, Rovani, Ilha et al
Anim Reprod (2019) 16 (4), 829-837
(3) "A study of Drynaria fortunei in modulation of BMP–2 signalling by bone tissue engineering"
Dong, Ma, Li et al
Turk J Med Sci (2020) 50 (5), 1444-1453
Showing 1-3 of 40 papers.