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Recombinant Human PLGF/PIGF2/PGF Protein, His Tag, 20µg  

Recombinant Human PLGF/PIGF2/PGF Protein, His Tag, 20µg

Recombinant Human PLGF / PGF Protein (rhPGF) Leu 19 - Arg 170 was produced in human 293 cells (HEK293), His tag

Synonym
Recombinant Human Protein, PGF, PLGF, PlGF2, PlGF, PGFL, SHGC-10760

More details

PGF-H5229-020

Availability: within 7 days

377,00 €

Background
Placental growth factor (PGF) also known as vascular endothelial growth factor-related protein, PLGF and PlGF2, is a member of the VEGF (vascular endothelial growth factor) sub-family - a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. The main source of PGF during pregnancy is the placental trophoblast. PGF is also expressed in many other tissues, including the villous trophoblast. PGF is actived in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. PlGF2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth.

Source
Recombinant Human PlGF Protein (rhPGF), His Tag (PGF-H5229) is expressed from human 293 cells (HEK293). It contains AA Leu 19 - Arg 170 (Accession # NP_002623.2).
Predicted N-terminus: Leu 19

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 18.2 kDa. The protein migrates as 28-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 100 mM Acetic Acid, pH3.0 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Sulfasalazine for the treatment of preeclampsia in a nitric oxide synthase antagonist mouse model"
Binder, de Alwis, Beard et al
Placenta (2023) 132, 20-26
(2) "Reduced expression in preterm birth of sFLT-1 and PlGF with a high sFLT-1/PlGF ratio in extracellular vesicles suggests a potential biomarker"
Hussein, Ju, Pizzella et al
Front Endocrinol (Lausanne) (2022) 13, 1024587
(3) "The Association of IL-17 and PlGF/sENG Ratio in Pre-Eclampsia and Adverse Pregnancy Outcomes"
Darmochwal-Kolarz, Chara
Int J Environ Res Public Health (2022) 20 (1)
Showing 1-3 of 2703 papers.