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Recombinant Human COMP /TSP5 Protein, 100µg  

Recombinant Human COMP /TSP5 Protein, 100µg

Recombinant Human COMP /TSP5 Protein (rh COMP) Gln 21 - Ala 757 was produced in human 293 cells (HEK293), His Tag

Synonym: recombinant, human, protein COMP,TSP5,EDM1,EPD1,MED,PSACH,THBS5

More details

COP-H5220-100

Availability: within 7 days

300,00 €

Background
Cartilage oligomeric matrix protein (COMP) is also known as Thrombospondin-5 (TSP5), EDM1, EPD1, MED, PSACH, THBS5, which belongs to the thrombospondin family. COMP / TSP5 contains 4 EGF-like domains, 1 TSP C-terminal (TSPC) domain, 8 TSP type-3 repeats. Abundantly expressed in the chondrocyte extracellular matrix, and is also found in bone, tendon, ligament and synovium and blood vessels. COMP may play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. COMP can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Thrombospondin-5 could play a role in the pathogenesis of osteoarthritis. COMP is a marker of cartilage turnover.

Source
Recombinant Human COMP /TSP5 Protein (rh COMP) Gln 21 - Ala 757 was produced in human 293 cells (HEK293).

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 82.0 kDa. The protein migrates as 110-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh COMP by the LAL method.

Purity
>88% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
N/A

References

(1) Chen F.-H., et al., 2006, J. Biol. Chem. 280:32655-32661.
(2) Koelling S., et al., 2006, Arthritis Res. Ther. 8:R56-R56.
(3) Gagarina V., et al., 2008, J. Biol. Chem. 283:648-659.
(4) Petersen, G., et al., 2009, Osteoarthritis and Cartilage 18 (1): 34–40.