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Recombinant Human PD-1 / PDCD1 Protein (HPLC-verified), His Tag, 100µg  

Recombinant Human PD-1 / PDCD1 Protein (HPLC-verified), His Tag, 100µg

Recombinant Human PD-1 / PDCD1 Protein Leu 25 - Gln 167 was produced in human 293 cells (HEK293), His Tag

Synonym
recombinant human protein PDCD1, PD1, CD279, SLEB2, hPD-1, hPD-l

More details

PD1-H5221-100

Availability: within 7 days

396,00 €

Background
Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.

Source
Recombinant Human PD-1 / PDCD1 Protein (Human PD-1, His Tag) Leu 25 - Gln 167 (Accession # NP_005009.2) was produced in human 293 cells (HEK293).

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus, and has a calculated MW of 16.8 kDa. The N-terminus Sequence Analysis is Leu 25. The reducing (R) protein migrates as 31-44 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 3 months (-20°C to -70°C).

Bioactivity
(1) Measured by its binding ability in a functional ELISA.
Immobilized Human PD-1, His Tag (Catalog # PD1-H5221) at 2 μg/mL (100 μL/well ) can bind Human PD-L1, Fc Tag (Catalog # PD1-H5258) with a linear range of 0.8-250 ng/mL.
(2) Measured by its binding ability in a functional ELISA.
Immobilized Human PD-1, His Tag (Catalog # PD1-H5221) at 0.2 μg/mL ( 100 μl/well ) can bind Human PD-L2, Fc Tag (Catalog # PD2-H5251) with a linear range of 0.16-2.5 μg/mL. 

References

(1) Ishida Y., et al., 1992, EMBO J. 11 (11): 3887–95.
(2) Blank C., et al., 2007, Cancer Immunol. Immunother. 56 (5): 739–45.
(3) Agata Y., et al., 1996, Int. Immunol. 8 (5): 765–72.
(4) Freeman GJ., et al., 2000, J. Exp. Med. 192 (7): 1027–34.
(5) Latchman Y., et al., 2001, Nat. Immunol. 2 (3): 261–8.
(6) Yamazaki T., et al., 2002, J. Immunol. 169 (10): 5538–45. (HEK293)

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