Recombinant Human Azurocidin /AZU1 Protein, 100µg

Background
Azurocidin (AZU1) is also known as Heparin-binding protein (HBP), Cationic antimicrobial protein CAP37, is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. which belongs to the peptidase S1 family and elastase subfamily. AZU1 / HBP contains 1 peptidase S1 domain. AZU1 binds heparin. The cytotoxic action of AZU1 is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. AZU1 may play a role in mediating recruitment of monocytes in the second wave of inflammation.

Source
Recombinant Human Azurocidin, His Tag (AZ1-H5225) is expressed from human 293 cells (HEK293). It contains AA Ile 27 - Pro 250 (Accession # AAH69495).
Predicted N-terminus: Ile 27

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 25.0 kDa. The protein migrates as 34-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "The Impact of Peri-implantitis on the Proteome Biology of Crevicular Fluid: A pilot study"
Halstenbach, Nelson, Iglhaut et al
J Periodontol (2022)
(2) "Increased CAP37 Expression in Stable Chronic Obstructive Pulmonary Disease"
Chen, Duan, Wang
Curr Med Sci (2022) 42 (5), 949-957
(3) "Clinical value of serum sTREM-1 and HBP levels in combination with traditional inflammatory markers in diagnosing hospital-acquired pneumonia in elderly"
Wang, Chang, Zhang et al
BMC Infect Dis (2022) 22 (1), 773
Showing 1-3 of 339 papers.