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Recombinant Human FZD2 /Frizzled-2 Protein, 100µg  

Recombinant Human FZD2 /Frizzled-2 Protein, 100µg

Recombinant Human FZD2 /Frizzled-2 Protein, with C-Fc Tag (rh FZD2 / Frizzled-2 Fc Chimera) Gln 24 - Ser 156 was produced in human 293 cells (HEK293).

Synonym: recombinant human protein, FZD2, Frizzled-2, Fz-2, hFz2 protein

More details

FZ2-H5251-100

Availability: within 7 days

348,00 €

Background
Frizzled-2 (FZD2) is also known as FzE2, which belongs to the G-protein coupled receptor Fz/Smo family. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. FZD2 contains one FZ (frizzled) domain. FZD2 may be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. The Lys-Thr-X-X-X-Trp motif of FZD2 interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.

Source
Recombinant Human FZD2 /Frizzled-2 Protein, with C-Fc Tag (rh FZD2 / Frizzled-2 Fc Chimera) Gln 24 - Ser 156 (Accession # AAI13403) was produced in human 293 cells (HEK293).

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 41.7 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh FZD2 / Frizzled-2 Fc Chimera by the LAL method.

Purity
>95% as determined by SDS-PAGE.

FormulationLyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

(1) Sagara N., et al., 1998, Biochem. Biophys. Res. Commun. 252:117-122.