Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Human TIM-3 / HAVCR2 Protein, 100µg  

Human TIM-3 / HAVCR2 Protein, 100µg

Recombinant Human TIM3 /HAVCR2 Protein, With C-Fc Tag (rhTIM3 / HAVCR2 Fc Chimera) Ser 22 - Arg 200 was produced in human 293 cells (HEK293).

Synonym: recombinant, human protein,  HAVCR2, TIM3, TIMD3, FLJ14428, KIM3

More details

TM3-H5258-100

Availability: within 7 days

348,00 €

Background
Hepatitis A virus cellular receptor 2 is also known as HAVCR2, FLJ14428, KIM3, TIM3, TIMD3, is a member of the TIM family of immune regulating molecules with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells and their associated cytokines are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. The 2 types of cells also cross-regulate the functions of the other. HAVCR2 is a Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice. HAVCR2 regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Dysregulation of the HAVCR2-galectin-9 pathway could underlie chronic autoimmune disease states in human, such as multiple sclerosis.

Source
Recombinant Human TIM3 /HAVCR2 Protein, With C-Fc Tag (rhTIM3 / HAVCR2 Fc Chimera) Ser 22 - Arg 200 (Accession # Q8TDQ0) was produced in human 293 cells (HEK293).

Molecular Characterization
rhTIM3 / HAVCR2 Fc Chimera, fused with Fc fragment of human IgG1 at the C-terminus, has a calculated MW of 46.5 kDa. The predicted N-terminus is Ser 22. As a result of glycosylation, DTT-reduced Protein migrates as 55-66 kDa and non-reduced protein migrates as 110-130.

Endotoxin
Less than 1.0 EU per μg of the rhTIM3 / HAVCR2 Fc Chimera by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Human TIM-3 (22-200), Fc Tag (Cat# TM3-H5258) at 2 μ g/mL (100 µl/well),can bind anti-TIM3 mAb with a linear range of 0.2-3 ng/mL.

References

(1) Monney, L. et al., 2002, Nature. 415: 536-41.
(2) Sabatos, CA. et al., 2003, Nat. Immunol. 4: 1102-10.
(3) Anderson, AC. et al., 2006, Curr. Opin. Immunol. 18: 665-9.