Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Human Nectin-2 / CD112 Protein, Fc Tag, 100µg  

Human Nectin-2 / CD112 Protein, Fc Tag, 100µg

Recombinant Human Nectin-2 / CD112 Protein, Poliovirus receptor-related 2 (PVRL2), Gln 32 - Leu 360 was produced in human 293 cells (HEK293), Fc Tag

Synonym: recombinant, human protein,  CD112, Nectin-2, PVRL2

More details

PV2-H5253-100

Availability: within 7 days

348,00 €

Background
Poliovirus receptor-related 2 (PVRL2) is also known as nectin-2 and CD112 (formerly herpesvirus entry mediator B, HVEB), which is a human plasma membrane glycoprotein. PVRL2 is one of the plasma membrane components of adherens junctions. PVRL2 also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in PVRL2 gene have been associated with differences in the severity of multiple sclerosis. Also, PVRL2 binds with low affinity to TIGIT. Furthermore, PVRL2 acts as a receptor for herpes simplex virus 1 (HHV-1) mutant Rid1, herpes simplex virus 1 (HHV-2) and pseudorabies virus (PRV).

Source
Recombinant Human Nectin-2 / CD112 Protein, Fc Tag, Gln 32 - Leu 360 (Accession # AAH03091) was produced in human 293 cells (HEK293)

Molecular Characterization
Human Nectin-2, Fc Tag is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 62.2 kDa. The predicted N-terminus is Gln 32. The reducing (R) protein migrates as 70-80 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). 

Bioactivity
Immobilized Human CD112, Fc Tag (Cat# PV2-H5253) at 10 μg/mL (100 μL/well) can bind Biotinylated Human TIGIT, His Tag (Catalog # TIT-H82E5) with a linear range of 0.6-5 μg/mL. 

References

(1) Martinez W.M., et al., 2001, J. Virol. 75:11185-11195.
(2) Yu X., et al., 2009, Nat. Immunol. 10:48-57.