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Recombinant Human VEGF R2 / KDR Protein, Strep Tag, 25µg  

Recombinant Human VEGF R2 / KDR Protein, Strep Tag, 25µg

Recombinant Human VEGF R2 / KDR Protein,  Ala 20 - Glu 764, expressed in human 293 cells, Strep Tag,

Synonym: recombinant human protein, KDR, CD309, FLK1, VEGFR, VEGFR2, kinase insert domain receptor

More details

KDR-H5280-25

Availability: within 7 days

312,00 €

Background
Kinase insert domain receptor (KDR) also known as CD309, FLK1, VEGFR, VEGFR2, and is one of the subtypes of VEGFR. VEGF receptors are receptors for vascular endothelial growth factor (VEGF). There are three main subtypes of VEGFR, numbered 1, 2 and 3. The VEGF receptors have an extracellular portion consisting of 7 immunoglobulin-like domains, a single transmembrane spanning region and an intracellular portion containing a split tyrosine-kinase domain. VEGF-A binds to VEGFR-1 (Flt-1) and VEGFR-2 (KDR/Flk-1). VEGFR-2 appears to mediate almost all of the known cellular responses to VEGF.The function of VEGFR-1 is less well defined, although it is thought to modulate VEGFR-2 signaling. Another function of VEGFR-1 may be to act as a dummy/decoy receptor, sequestering VEGF from VEGFR-2 binding (this appears to be particularly important during vasculogenesis in the embryo).[1-3] In addition, VEGFR2 is able to interact with HIV-1 extracellular Tat protein upon VEGF activation, and seems to enhance angiogenesis in Kaposi\'s sarcoma lesions.

Source
Recombinant Human VEGF R2, Strep Tag (KDR-H5280) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Glu 764 (Accession # AAI31823).
Predicted N-terminus: Ala 20

Molecular Characterization
Recombinant Human VEGF R2, Strep Tag is fused with twin strep tag at the C-terminus, and has a calculated MW of 86.3 kDa. The predicted N-terminus is Ala 20. The reducing (R) protein migrates as 100-110 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4C-8C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Immobilized ActiveMax® Human VEGF121 (Catalog # VE1-H4213) at 2 μg/mL can bind Human VEGF R2, Strep Tag (Catalog # KDR-H5280) with a linear range of 0.15-2.5 μg/mL.

References

(1) Holmes K,et al. (2007) . Cell Signal. 19 (10): 2003–2012.
(2) Stuttfeld E, et al. (2009). IUBMB Life 61 (9): 915–22.
(3) Fujita, N, et al. (2008). Biochemical and biophysical research communications 372 (2): 367–372.

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