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Recombinant Human FGL1 Protein, Fc Tag, 100 µg  

Recombinant Human FGL1 Protein, Fc Tag, 100 µg

Recombinant Human FGL1 Protein, AA Leu 23 - Ile 312, expressed from human 293 cells, Fc Tag

Synonym: Recombinant human protein, FGL1, Hepassocin, HP-041, HFREP-1, LFIRE-1, HFREP1

More details

FG1-H5258-100

Availability: within 7 days

420,00 €

Background
Fibrinogen-like protein 1(FGL1) is also known as HP-041, Hepassocin, HFREP-1, LFIRE-1. The protective effect of fibrinogen-like protein 1 (FGL1) in liver injury has previously been reported. However, studies have shown that FGL1 may be a predictor of GC patients and a target for GC therapy. Immunocytochemical studies revealed that fgl1 selectively binds to defective spermatozoa in the cauda epididymidis. Northern blot analysis and in situ hybridization demonstrated the high expression of fgl1 in the principal cells of the proximal cauda epididymidis. Immunofluorescence analysis using mouse fibrotic lung tissues suggested that fibrotic regions showed increased expressions of Gtse1 and Fgl1, Gtse1 and Fgl1 are suggested to be novel targets for radiation-induced lung fibrosis.

Source
Recombinant Human FGL1, Fc Tag (FG1-H5258) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Ile 312 (Accession # Q08830-1).
Predicted N-terminus: Pro

Molecular Characterization
This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 60.5 kDa. The protein migrates as 60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity-ELISA
Immobilized Human LAG-3, Mouse IgG2a Fc Tag (Cat. No. LA3-H52Aa) at 10 μg/mL (100 μL/well) can bind Human FGL1, Fc Tag (Cat. No. FG1-H5258) with a linear range of 0.02-0.156 μg/mL (QC tested).

Bioactivity-BLI
Loaded Human LAG-3, Mouse IgG2a Fc Tag (Cat. No. LA3-H52Aa) on AMC Biosensor, can bind Human FGL1, Fc Tag (Cat. No.FG1-H5258) with an affinity constant of 43.4 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-FACS
FACS assay shows that Human FGL1, Fc Tag (Cat. No. FG1-H5258) can bind to 293T cells overexpressing human LAG3. The concentration of Human FGL1 is 3 μg/mL (Routinely tested).

References

(1)  Nagdas SK. 2016. Int J Biochem Cell Biol. 80:163-172.
(2)  Jin H. 2018 Nov 7. J Mol Med (Berl).
(3)  Zhang Y. 2018 Aug. Mol Med Rep. 18(2):1465-1472.