Recombinant Human Mucin-1 / MUC-1 Protein, Llama IgG2b Fc Tag, low endotoxin, 100 µg

Background
Membrane mucins have several functions in epithelial cells including cytoprotection, extravasation during metastases, maintenance of luminal structure, and signal transduction. MUC17, contains an extended, repetitive extracellular glycosylation domain and a carboxyl terminus with two EGF-like domains, a SEA module domain, a transmembrane domain, and a cytoplasmic domain with potential serine and tyrosine phosphorylation sites. Interacts via its C-terminus with PDZK1 and this interaction appears important for proper localization. Probably plays a role in maintaining homeostasis on mucosal surfaces.

Source
Recombinant Human Mucin-1, Llama IgG2b Fc Tag, low endotoxin (MU1-H5255) is expressed from human 293 cells (HEK293). It contains AA Ser 33 - Gly 167 (Accession # AAI20976).
Predicted N-terminus: Ser 33

Molecular Characterization
This protein carries a llama IgG2b Fc tag at the C-terminus.
The protein has a calculated MW of 42.4 kDa. The protein migrates as 50-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 0.01 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1)  "MUC1 promotes cervical squamous cell carcinoma through ERK phosphorylation-mediated regulation of ITGA2/ITGA3"
Zhao, Pan, Gao et al
BMC Cancer (2024) 24 (1), 559
(2)  "HMUC1 aptamer-tethered H40-TEPA-PEG nanoconjugates for targeted siRNA-delivery and gene silencing in breast cancer cells"
Salve, Haldar, Shaikh et al
Front Bioeng Biotechnol (2024) 12, 1383495
(3)  "Multivariate logistic regression analysis of poor prognosis of dermatomyositis and clinical value of ferritin/Kl-6 in predicting prognosis"
Yan, Shi, Wu et al
Skin Res Technol (2024) 30 (5), e13701
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