Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Human IL-7 R alpha / CD127 Protein, Mouse IgG2a Fc Tag, 100 µg  

Recombinant Human IL-7 R alpha / CD127 Protein, Mouse IgG2a Fc Tag, 100 µg

Recombinant Human IL-7 R alpha / CD127 Protein, AA Glu 21 - Gly 236, expressed from human 293 cells (HEK293), Mouse IgG2a Fc Tag

Synonym: Recombinant, human, protein, IL7Ra, CD127

More details

IL7-H5258-100

Availability: within 7 days

416,00 €

Background
Interleukin-7 receptor subunit alpha (IL7Ra) is also known as CD antigen CD127, which belongs to the type I cytokine receptor family and type 4 subfamily. IL7Ra /CD127 contains 1 fibronectin type-III domain. IL7Ra /CD127 is expressed on various cell types, including naive and memory T cells and many others. IL7Ra /CD127 is receptor for interleukin-7 and also acts as a receptor for thymic stromal lymphopoietin (TSLP). The IL7 receptor is a heterodimer of IL7R and IL2RG. The TSLP receptor is a heterodimer of CRLF2 and IL7R.

Source
Recombinant Human IL-7 R alpha, Mouse IgG2a Fc Tag (IL7-H5258) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Gly 236 (Accession # P16871-1(I66T, V138I)).
Predicted N-terminus: Glu 21

Molecular Characterization
This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 52.0 kDa. The protein migrates as 65-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Plasma cytokines during pregnancy provide insight into the risk of diabetes in the gestational diabetes risk group"
Tagoma, Haller-Kikkatalo, Oras et al
J Diabetes Investig (2022)
(2) "Peripheral inflammatory cytokines and motor symptoms in persons with Parkinson's disease"
Diaz, Kohut, Russell et al
Brain Behav Immun Health (2022) 21, 100442
(3) "Honey Bee Products: Preclinical and Clinical Studies of Their Anti-inflammatory and Immunomodulatory Properties"
El-Seedi, Eid, Abd El-Wahed et al
Front Nutr (2021) 8, 761267
Showing 1-3 of 155 papers.