Recombinant Human Galectin-3 Protein, His Tag, 100 µg

Background
Galectin-3 (Gal-3) is also known as LGALS3, 35 kDa lectin, Carbohydrate-binding protein 35 (CBP 35), Galactose-specific lectin 3, Galactoside-binding protein (GALBP), IgE-binding protein, Laminin-binding protein, Mac-2 antigen, Lectin L-29. Galectin-3 is a member of the lectin family. LGALS3 / Galectin-3 is expressed in the nucleus, cytoplasm, mitochondrion, cell surface, and extracellular space. LGALS3 / Galectin-3 has been shown to be involved in the following biological processes: cell adhesion, cell activation and chemoattraction, cell growth and differentiation, cell cycle, and apoptosis.

Source
Recombinant Human Galectin-3 protein, His Tag (GA3-H5247) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ile 250 (Accession # P17931-1).
Predicted N-terminus: His

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 27.5 kDa. The protein migrates as 35-41 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM HEPES, 150 mM NaCl, 5 mM EDTA, 2 mM TCEP, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1)  "Cannabidiol inhibits lung proliferation in monocrotaline-induced pulmonary hypertension in rats"
Krzyżewska, Baranowska-Kuczko, Kasacka et al
Biomed Pharmacother (2023) 159, 114234
(2)  "Efficacy of Serum Apelin and Galectin-3 as Potential Predictors of Mortality in Severe COVID-19 Patients"
Berber, Geçkil, Altan et al
J Med Virol (2023)
(3)  "YAP-galectin-3 signaling mediates endothelial dysfunction in angiotensin II-induced hypertension in mice"
Pang, Sun, Bai et al
Cell Mol Life Sci (2023) 80 (2), 38
Showing 1-3 of 5214 papers.