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Recombinant Human TNFR1 / CD120a / TNFRSF1A Protein, His Tag, 100 µg  

Recombinant Human TNFR1 / CD120a / TNFRSF1A Protein, His Tag, 100 µg

Recombinant Human TNFR1 / CD120a / TNFRSF1A Protein, AA Ile 22 - Thr 211, expressed from human 293 cells (HEK293), His Tag

Synonym: Recombinant, human, protein, TNFR1, CD120a, TNFRSF1A, FPF, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR55, TNFR60, p55, p60

More details

TN1-H5222-100

Availability: within 7 days

325,00 €

Background
Tumor necrosis factor receptor 1 (TNF-R1) is also known as Tumor necrosis factor receptor superfamily member 1A (TNFRSF1A), TNFAR, CD antigen CD120a, which belongs to the tumor necrosis factor receptor superfamily. TNF-R1 contains one death domain and four TNFR-Cys repeats. TNF-R1 is the receptor of TNFSF2 / TNF-alpha and homotrimeric TNFSF1 / lymphotoxin - alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. TNF-R1 contributes to the induction of non - cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Defects in TNFRSF1A are the cause of familial hibernian fever (FHF).

Source
Recombinant Human TNFR1 protein, His Tag (TN1-H5222) is expressed from human 293 cells (HEK293). It contains AA Ile 22 - Thr 211 (Accession # NP_001056).
Predicted N-terminus: Ile 22

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 22.3 kDa. The protein migrates as 28-40 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1)  "SPATA2 restricts OTULIN-dependent LUBAC activity independently of CYLD"
Griewahn, Müller, Peintner et al
Cell Rep (2023) 42 (1), 111961
(2)  "Synovial macrophages of rheumatoid arthritic mice protectively responded by altered M1/M2 differentiation after antibody blocking of TNFR1 and IL-1R"
Kanwar, Dey, Maiti et al
Int Immunopharmacol (2023) 115, 109654
(3)  "Genetically proxied inhibition of tumor necrosis factor and the risk of colorectal cancer: A drug-target mendelian randomization study"
Chen, Chen, Xiao et al
Front Pharmacol (2022) 13, 1079953
Showing 1-3 of 6556 papers.