Recombinant Human PSGL-1 / CD162 Protein, His Tag, 100 µg

Background
P-selectin glycoprotein ligand 1 (PSGL-1) is also known as Selectin P ligand (SELPLG), CD antigen CD162. PSGL-1 is disulfide-linked homodimer which is the high affinitycounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 / SELPLG interacts with P-, E- and L-selectins, through their lectin/EGF domains, is required for promoting recruitment and rolling of leukocytes. These interactions require sialyl Lewis X glycan modification but there is a differing dependence for tyrosine sulfations. Dimerization appears not to be required for P-selectin/SELP binding. PSGL-1 can interact with SNX20, MSN and SYK. PSGL-1 / SELPLG mediate the activation of SYK by SELPLG.

Source
Recombinant Human PSGL-1, His Tag (PS1-H52H9) is expressed from human 293 cells (HEK293). It contains AA Gln 42 - Cys 320 (Accession # Q14242-1).
Predicted N-terminus: Gln 42

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 30.7 kDa. The protein migrates as 55-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Early SARS-CoV-2 infection: platelet-neutrophil complexes and platelet function"
Rieder, Baldus, Stallmann et al
Res Pract Thromb Haemost (2022)
(2) "Abacavir causes leukocyte/platelet crosstalk by activating neutrophil P2X7 receptors thus releasing soluble lectin-like oxidized low-density lipoprotein receptor-1"
Blanch-Ruíz, Sánchez-López, Ríos-Navarro et al
Br J Pharmacol (2022)
(3) "Immunophenotyping of Circulating and Intratumoral Myeloid and T Cells in Glioblastoma Patients"
Marx, Wilken, Miebach et al
Cancers (Basel) (2022) 14 (23)
Showing 1-3 of 953 papers.