Recombinant Mouse IL-3 R alpha / CD123 Protein, His Tag (SPR verified), 100 µg

Background
Interleukin 3 receptor alpha (low affinity) (IL3RA), also known as CD123 (Cluster of Differentiation 123) is a 70-kD glycoprotein member of the hematopoietin receptor superfamily. This protein associates with a beta subunit common to the receptors for IL-5 and granulocyte-macrophage colony-stimulating factor (GM-CSF) to form a high-affinity receptor for IL-3. The interleukin-3 receptor α chain (CD123) has been identified as a potential immunotherapeutic target because it is overexpressed in AML compared with normal hematopoietic stem cells.

Source
Recombinant Mouse IL-3 R alpha, His Tag (ILA-M52H4) is expressed from human 293 cells (HEK293). It contains AA Ser 17- Lys 331 (Accession # P26952-1).
Predicted N-terminus: Ser 17

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 36.4 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1)"A phase I/II study of tagraxofusp in Japanese patients with blastic plasmacytoid dendritic cell neoplasm
Yokota, Munakata, Kiguchi et al
Int J Hematol (2026)
(2) "Hematopoietic effects of tagraxofusp in treatment-naive patients with blastic plasmacytoid dendritic cell neoplasm
Konopleva, Pemmaraju, Sweet et al
Cancer (2026) 132 (1), e70243
(3) "IL-3/STAT5/miR-155-5p axis supports stem-related pathway reprogramming in TNBC
Sarcinella, Kholia, Femminò et al
Breast Cancer Res (2025) 27 (1), 195
Showing 1-3 of 180 papers.