Recombinant Human Siglec-10 Protein, His Tag (MALS verified), 100 µg

Background
The siglecs (sialic acid-binding Ig-like lectins) are a distinct subset of the Ig superfamily with adhesion-molecule-like structure. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.

Source
Recombinant Human Siglec-10, His Tag (SI0-H52H9) is expressed from human 293 cells (HEK293). It contains AA Met 17 - Thr 546 (Accession # Q96LC7-1).
Predicted N-terminus: Met 17

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 60.1 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in 25 mM MES, 150 mM NaCl, Arginine, pH5.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Role of inhibitory B cell co-receptors in B cell self-tolerance to non-protein antigens"
Tsubata
Immunol Rev (2022)
(2) "Elevated CD19+Siglec-10+ B cell levels are correlated with systemic lupus erythematosus disease activity"
Ju, Wang, Mo et al
Int Immunopharmacol (2022) 102, 108403
(3) "Dried Blood Specimens as an Alternative Specimen for Immune Response Monitoring During HIV Infection: A Proof of Concept and Simple Method in a Pediatric Cohort"
Rubio-Garrido, Avendaño-Ortiz, Ndarabu et al
Front Med (Lausanne) (2021) 8, 678850
Showing 1-3 of 54 papers.