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Recombinant Mouse VEGF R1 / Flt-1 Protein, Mouse IgG2a Fc Tag, low endotoxin, 100µg  

Recombinant Mouse VEGF R1 / Flt-1 Protein, Mouse IgG2a Fc Tag, low endotoxin, 100µg

Recombinant Mouse VEGF R1 / Flt-1 Protein, AA Tyr 23 - Glu 759, produced in human 293 cells (HEK293), mouse IgG2a Fc Tag, low endotoxin

Synonym: recombinant human protein, FLT, VEGFR1, FLT1

More details

VE1-M5256-100

Availability: within 7 days

360,00 €

Background
Vascular endothelial growth factor receptor 1 (VEGFR1) also known as Fms-like tyrosine kinase 1 (FLT-1), Tyrosine-protein kinase receptor FLT, is a single-pass type I membrane protein and secreted protein which belongs to the protein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily. VEGFR1 is detected in normal lung, but also in placenta, liver, kidney, heart and brain tissues and specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. VEGFR1 acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. VEGFR1 may play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. VEGFR1 can promote endothelial cell proliferation, survival and angiogenesis in adulthood.

Source
Recombinant Mouse VEGF R1 / FLT-1 protein, Mouse IgG2a Fc Tag, low endotoxin (VE1-M5256) is expressed from human 293 cells (HEK293). It contains AA Tyr 23 - Glu 759 (Accession # P35969-1).
Predicted N-terminus: Tyr 23

Molecular Characterization
This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 109.8 kDa. The protein migrates as 140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 0.1 EU per μg by the LAL method.

Purity
>85% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
    -20°C to -70°C for 12 months in lyophilized state;
    -70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.