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Recombinant Biotinylated Human Growth Hormone R, ultra sensitivity (primary amine labeling), His-Tag, 50 µg  

Recombinant Biotinylated Human Growth Hormone R, ultra sensitivity (primary amine labeling), His-Tag, 50 µg

Recombinant Biotinylated Human Growth Hormone R (GHR), expressed from human HEK293 cells, His-Tag

Synonyms: recombinant, human, biotinylated, protein, GHR,GHBP,GH receptor

More details

GHR-H8222-50

492,00 €

Background
Growth hormone receptor (GHR) is also known as somatotropin receptor, growth hormone-binding protein (GHBR), which belongs to the type I cytokine receptor family or Type 1 subfamily. GHR contains one fibronectin type-III domain. GHR / GHBR is expressed in various tissues with high expression in liver and skeletal muscle. The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. GHR is receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.

Source
MABSol® Biotinylated Human GHR, His Tag, ultra sensitivity (primary amine labeling) (GHR-H8222) is expressed from human HEK293 cells. It contains AA Ala 27 - Tyr 264 (Accession # P10912-1). It is the biotinylated form of Human Growth Hormone R (GHR) Protein (GHR-H5222).
Predicted N-terminus: Ala 27

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 29.6 kDa. The protein migrates as 40-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by reduced SDS-PAGE.

Bioactivity
Immobilized Human GH, Tag Free (Cat. No. GH1-H4117) at 2 μg/mL (100 μL/well) can bind Biotinylated Human GHR, His Tag, ultra sensitivity (primary amine labeling) (Cat. No. GHR-H8222) with a linear range of 0.5-8 ng/mL (QC tested).

Formulation
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4) with Trehalose as protectant.
Contact us for customized product form or formulation.

Reconstitution
Reconstitute at 100 μg/mL in sterile deionized water. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the COA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

References

(1) Duquesnoy P., et al., 1994, EMBO J. 13:1386-1395.
(2) Urbanek M., et al., 1993, J. Biol. Chem. 268:19025-19032.