Products

Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Biotinylated Human Mesothelin / MSLN (296-580), Fc Tag, ultra sensitivity (primary amine labeling), Fc Tag, 25µg  

Recombinant Biotinylated Human Mesothelin / MSLN (296-580), Fc Tag, ultra sensitivity (primary amine labeling), Fc Tag, 25µg

MABSol® Recombinant Biotinylated Human Mesothelin / MSLN (296-580), expressed from human HEK293 cells, Fc Tag, ultra sensitivity (primary amine labeling) (MALS verified)

Synonym
recombinant, human, biotinylated, protein, MSLN,Mesothelin,MPF

More details

MSN-H826x-25

Availability: within 7 days

348,00 €

Background
Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.

Source
Recombinant MABSol® Biotinylated Human Mesothelin (296-580), c Tag (MSN-H826x) is expressed from human HEK293 cells. It contains AA Glu 296 - Gly 580 (Accession # AAH09272). It is the biotinylated form of Human Mesothelin (296-580), Fc Tag (MSN-H526x).

Molecular Characterization
This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 59.1 kDa. The protein migrates as 60-68 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

Biotin:Protein Ratio
The biotin to protein ratio is 3-5 as determined by the HABA assay.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the COA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "ASO Visual Abstract: Mesothelin Expression is Correlated with Chemoresistance in Stage IV Colorectal Cancer"
Nagata, Shinto, Shiraishi et al
Ann Surg Oncol (2021)
(2) "Phase I study of CAR-T cells with PD-1 and TCR disruption in mesothelin-positive solid tumors"
Wang, Li, Feng et al
Cell Mol Immunol (2021)
(3) "Therapeutic Targeting of Mesothelin with Chimeric Antigen Receptor T Cells in Acute Myeloid Leukemia"
Le, Castro, Tang et al
Clin Cancer Res (2021)
Showing 1-3 of 1112 papers.
Please refer to product data sheet.