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Recombinant Biotinylated Human OX40, Fc Tag, Avi Tag (Avitag™), 25µg  

Recombinant Biotinylated Human OX40, Fc Tag, Avi Tag (Avitag™), 25µg

MABSol® Recombinant Biotinylated Human OX40 / TNFRSF4 / CD134, expressed from human HEK293 cells, Fc-Tag, Avi tag (Avitag™)

Synonym
recombinant, human, biotinylated, protein TNFRSF4,OX40,CD134,OX40L receptor

More details

OX0-H82F7-25

Availability: within 7 days

420,00 €

Background
Tumor necrosis factor receptor superfamily member 4 (TNFRSF4) is also known as ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, CD antigen CD134, OX40. OX40 / TNFRSF4 contains four TNFR-Cys repeats. TNFRSF4 is receptor for TNFSF4 / OX40L / GP34 and can interacts with TRAF2, TRAF3 and TRAF5.

Source
Biotinylated Human OX40, Fc,Avitag (OX0-H82F7) is expressed from human 293 cells (HEK293). It contains AA Leu 29 - Ala 216 (Accession # NP_003318).
Predicted N-terminus: Leu 29

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 48.6 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Reconstitute at 100 μg/mL in sterile deionized water. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the COA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Bioactivity-ELISA
Immobilized Human OX40 Ligand, Fc Tag (Cat. No. OXL-H526x) at 5 μg/mL (100 μL/well) can bind Biotinylated Human OX40, Fc,Avitag (Cat. No. OX0-H82F7) with a linear range of 5-78 ng/mL (QC tested).

Limited Use License
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrosbiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.
Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

References

(1) Arch R.H., et al., 1998, Mol. Cell. Biol. 18:558-565.
(2) Kawamata S., et al., 1998, J. Biol. Chem. 273:5808-5814.