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Recombinant Biotinylated Human Transferrin R/CD71, ultra sensitivity (primary amine labeling), His-Tag, 25µg  

Recombinant Biotinylated Human Transferrin R/CD71, ultra sensitivity (primary amine labeling), His-Tag, 25µg

MABSol® Recombinant Biotinylated Human Transferrin R/CD71 Protein (TFR-H8243), AA Cys 89 - Phe 760, expressed from human HEK293 cells, His-Tag, ultra sensitivity (primary amine labeling)

Synonym
recombinant, human, biotinylated, protein, CD71, TFRC, TFR, sTfR, TfR1, Trfr, T9, p90

More details

TFR-H8243-25

Availability: within 7 days

348,00 €

Background
CD antigen CD71 is also known as Transferrin receptor protein 1, TfR, sTfR, p90, TfR1, Trfr, which belongs to the peptidase M28 family and M28B subfamily. CD71 /TFR contains one PA (protease associated) domain. CD71 / TfR1 is required for iron delivery from transferrin to cells. CD71 is a potential new target in cases of human leukomia & lymphoma. CD71 /TFRC / TfR has been shown to interact with GABARAP and HFE.

Source
Recombinant Biotinylated Human Transferrin R, His Tag, primary amine labeling is expressed from human HEK293 cells. It contains AA Cys 89 - Phe 760 (Accession # AAH01188.1). It is the biotinylated form of Human Transferrin R protein, His Tag (Cat. No. CD1-H5243).
Predicted N-terminus: His

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 76.0 kDa. The protein migrates as 75-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

Biotin:Protein Ratio
Passed as determined by the HABA assay / binding ELISA.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Transferrin Protein Corona-Modified CuGd Core-Shell Nanoplatform for Tumor-Targeting Photothermal and Chemodynamic Synergistic Therapies"
Qi, Wang, Wang et al
ACS Appl Mater Interfaces (2022)
(2) "Depletion of mmu_circ_0001751 (circular RNA Carm1) protects against acute cerebral infarction injuries by binding with microRNA-3098-3p to regulate acyl-CoA synthetase long-chain family member 4"
Mao, Liu
Bioengineered (2022) 13 (2), 4063-4075
(3) "Caffeine and alcohol - Friends or foes of human iron stores?"
Dziembowska, Wójcik, Żekanowska
J Trace Elem Med Biol (2022) 71, 126922
Showing 1-3 of 2330 papers.