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Recombinant Biotinylated Mouse IgG2a Fc, Avi Tag (Avitag™), 25 µg  

Recombinant Biotinylated Mouse IgG2a Fc, Avi Tag (Avitag™), 25 µg

Recombinant Biotinylated Mouse IgG2a Fc Protein, AA Glu 98 - Lys 330, expressed from human HEK293 cells, Avi Tag (Avitag™) (MALS verified)

Synonyms: recombinant, mouse, biotinylated, protein, IgG2A

More details

IGA-M8210-25

432,00 €

Background
Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b. It was found that IgG2a was superior to IgG1 in activating complement. The glycosylation of the circulating immunoglobulin-? (IgG) antibody molecules changes in rheumatoid arthritis.

Source
Recombinant Biotinylated Mouse IgG2a Fc, Avitag (IGA-M8210) is expressed from human 293 cells (HEK293). It contains AA Glu 98 - Lys 330 (Accession # P01863).
Predicted N-terminus: Glu 98

Molecular Characterization
This protein carries an Avi tag at the C-terminus.
The protein has a calculated MW of 28.2 kDa. The protein migrates as 32-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein ratio
The biotin to protein ratio is 0.7-1 as determined by the HABA assay

Endotoxin
Less than 1.0 EU per µg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Imlifidase-generated Single-cleaved IgG: Implications for Transplantation"
Bockermann, Järnum, Runström et al
Transplantation (2021)
(2) "Antibody-free profiling of transcription factor occupancy during early embryogenesis by FitCUT&RUN"
Wang, Wang, Wang et al
Genome Res (2021)
(3) "Unexplored Excipients in Biotherapeutic Formulations: Natural Osmolytes as Potential Stabilizers Against Thermally Induced Aggregation of IgG1 Biotherapeutics"
Bhojane, Joshi, Sahoo et al
AAPS PharmSciTech (2021) 23 (1), 26
Showing 1-3 of 10115 papers.

Limited Use License
The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrosbiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.
Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.