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Recombinant Biotinylated Human TIGIT Protein, Fc Tag, Avi Tag (Avitag™), 25µg  

Recombinant Biotinylated Human TIGIT Protein, Fc Tag, Avi Tag (Avitag™), 25µg

MABSol® Biotinylated Recombinant Human TIGIT Protein, expressed from human HEK293 cells (AA Met 22 - Pro 141), Fc Tag, Avi tag (Avitag™(MALS verified))

Synonym
recombinant, human, biotinylated, protein,TIGIT,VSIG9,VSTM3

More details

TIT-H82F1-25

Availability: within 7 days

420,00 €

Background
T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

Source
Recombinant Biotinylated Human TIGIT, Fc,Avitag (MALS verified) (TIT-H82F1) is expressed from human 293 cells (HEK293). It contains AA Met 22 - Pro 141 (Accession # Q495A1-1).
Predicted N-terminus: Met 22

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).
The protein has a calculated MW of 41.3 kDa. As a result of glycosylation, the protein migrates as 45-55 kDa under reducing (R) condition, and 85-115 kDa under non-reducing (NR) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Biotin:Protein Ratio
The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

Bioactivity
Please refer to product data sheet.

References

(1) Yu X., et al., 2009, Nat. Immunol. 10:48-57.
(2) Stengel K.F., et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109:5399-5404.
(3) Lozano E., et al., 2012, J. Immunol. 188:3869-3875.


Limited Use License
The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrosbiosystems’s AviTagTM proteins confers to the purchaser the limited right to use the AviTagTM technology for non-commercial, or research use, or for purposes of evaluating the AviTagTM technology.
Commercial use of the AviTagTM technology to manufacture a commercial product, or use of the AviTagTM technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.