Recombinant Biotinylated Human B7-H5 / Gi24 / VISTA, Avi Tag (Avitag™), His Tag, 25µg

Background
Platelet receptor Gi24, also known as B7-H5 and stress-induced secreted protein-1 (Sisp-1), is a protein that in humans is encoded by the C10orf54 gene, which contains 1 Ig-like (immunoglobulin-like) domain. As for C10orf54 gene, C10orf54 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state on ESCs. Human C10orf54 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment.

Source
Recombinant Biotinylated Human B7-H5, Avitag,His Tag (B75-H82E1) is expressed from human 293 cells (HEK293). It contains AA Phe 33 - Ala 194 (Accession # AAH20568.1).
Predicted N-terminus: Phe 33

Molecular Characterization
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 20.8 kDa. The protein migrates as 36-47 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio
The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Comprehensive characterization of B7 family members in NSCLC and identification of its regulatory network"
Xiao, Pang, Xiang et al
Sci Rep (2023) 13 (1), 4311
(2) "B7-H4 Further Stratifies Patients With Endometrial Cancer Exhibiting a Nonspecific Molecular Profile"
Zong, Yu, Mo et al
Arch Pathol Lab Med (2023)
(3) "Novel immune scoring dynamic nomograms based on B7-H3, B7-H4, and HHLA2: Potential prediction in survival and immunotherapeutic efficacy for gallbladder cancer"
Lv, Han, Wu et al
Front Immunol (2022) 13, 984172
Showing 1-3 of 33 papers.

Citations

(1) "VISTA is an acidic pH-selective ligand for PSGL-1."
Authors: Johnston RJ, Su LJ, Pinckney J, et al.
Journal: Nature 2019
Application: Flow cytometry