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Recombinant Biotinylated Human B7-H5 / Gi24 / VISTA, Avi Tag (Avitag™), His Tag, 25µg  

Recombinant Biotinylated Human B7-H5 / Gi24 / VISTA, Avi Tag (Avitag™), His Tag, 25µg

MABSol® Biotinylated Recombinant Human B7-H5 / Gi24 / VISTA Protein, expressed from human HEK293 cells, (AA Phe 33 - Ala 194), Avi tag (Avitag™), His Tag

Synonym
recombinant, human, biotinylated, protein, B7-H5,SISP1,Gi24,VISTA

More details

B75-H82E1-25

Availability: within 7 days

420,00 €

Background
Platelet receptor Gi24, also known as B7-H5 and stress-induced secreted protein-1 (Sisp-1), is a protein that in humans is encoded by the C10orf54 gene, which contains 1 Ig-like (immunoglobulin-like) domain. As for C10orf54 gene, C10orf54 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state on ESCs. Human C10orf54 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment.

Source
Biotinylated Human B7-H5, Avitag,His Tag (B75-H82E1) is expressed from human 293 cells (HEK293). It contains AA Phe 33 - Ala 194 (Accession # AAH20568.1).
Predicted N-terminus: Phe 33

Molecular Characterization
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 20.8 kDa. The protein migrates as 36-47 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Biotin:Protein Ratio
The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
 In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

References

(1) Sakr M.A., et al., 2010, Cancer Sci. 101:2368-2374.
(2) Mayya V., et al ., 2009, Sci. Signal. 2:RA46-RA46.

Limited Use License
The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrosbiosystems’s AviTagTM proteins confers to the purchaser the limited right to use the AviTagTM technology for non-commercial, or research use, or for purposes of evaluating the AviTagTM technology.

Commercial use of the AviTagTM technology to manufacture a commercial product, or use of the AviTagTM technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.