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Recombinant Biotinylated Human BAFF / TNFSF13B / CD257 Protein, Avi Tag (Avitag™), 25µg  

Recombinant Biotinylated Human BAFF / TNFSF13B / CD257 Protein, Avi Tag (Avitag™), 25µg

MABSol® Recombinant Biotinylated Human BAFF / TNFSF13B / CD257 Protein, Ala 134 - Leu 285, expressed from human 293 cells (HEK293), His Tag, Avi tag (Avitag™) (active trimer) (MALS verified)

Synonym
recombinant, human, biotinylated, protein, TNFSF13B, BAFF, BLYS, CD257, DTL, TALL-1, TALL1, THANK, TNFSF20, ZTNF4

More details

BAF-H82Q2-25

Availability: within 7 days

455,00 €

Background
B-cell activating factor (BAFF) is also known as tumor necrosis factor ligand superfamily member 13B , TNFSF13B, BAFF, B Lymphocyte Stimulator (BLyS) , cluster of differentiation 257 (CD257), DTL, TNF- and APOL-related leukocyte expressed ligand (TALL-1), THANK, TNFSF20, ZTNF4, and is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. It is expressed as transmembrane protein on various cell types including monocytes, dendritic cells and bone marrow stromal cells. BAFF is the natural ligand of three unusual tumor necrosis factor receptors named BAFF-R, TACI, and BCMA, all of which have differing binding affinities for it. These receptors are expressed mainly on mature B lymphocytes (TACI is also found on a subset of T-cells and BCMA on plasma cells). TACI binds worst since its affinity is higher for a protein similar to BAFF, called a proliferation-inducing ligand (APRIL). BCMA displays an intermediate binding phenotype and will work with either BAFF or APRIL to varying degrees. Signaling through BAFF-R and BCMA stimulates B lymphocytes to undergo proliferation and to counter apoptosis. All these ligands act as heterotrimers (i.e. three of the same molecule) interacting with heterotrimeric receptors, although BAFF has been known to be active as either a hetero- or homotrimer. BAFF acts as a potent B cell activator and has been shown to play an important role in the proliferation and differentiation of B cells.

Source
Recombinant Biotinylated Human BAFF, His,Avitag (active trimer) (MALS verified) (BAF-H82Q2) is expressed from human 293 cells (HEK293). It contains AA Ala 134 - Leu 285 (Accession # AAH20674.1).
Predicted N-terminus: His

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 20.2 kDa. The protein migrates as 21-23 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio
Passed as determined by the HABA assay / binding ELISA.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "HSPB5 suppresses renal inflammation and protects lupus-prone NZB/W F1 mice from severe renal damage"
Knapp, Braunstein, Berg et al
Arthritis Res Ther (2022) 24 (1), 267
(2) "TNFSF13B rs9514828 gene polymorphism and soluble B cell activating factor levels: association with apical periodontitis"
Cruz, Gascón, Palafox-Sánchez et al
Int Endod J (2022)
(3) "Cytokine and chemokine multiplex analysis-based exploration for potential treatment and prognostic prediction in large-vessel vasculitis: A preliminary observational study"
Abe, Kono, Kono et al
Front Immunol (2022) 13, 1066916
Showing 1-3 of 2557 papers.