kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Biotinylated Human B7-H4 Protein, Avitag™,His Tag (recommended for biopanning), 25µg  

Recombinant Biotinylated Human B7-H4 Protein, Avitag™,His Tag (recommended for biopanning), 25µg

Biotinylated Human B7-H4 Protein, VTCN1 (recommended for biopanning), expressed from human 293 cells (HEK293), Avitag™,His Tag (recommended for biopanning)

recombinant, human, biotinylated, protein, B7-H4, VTCN1, B7S1, B7h.5

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Availability: within 7 days

481,00 €

V-set domain-containing T-cell activation inhibitor 1 (VTCN1) is also known as Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, B7H4, which belongs to the immunoglobulin superfamily and BTN/MOG family. VTCN1 contains two Ig-like V-type (immunoglobulin-like) domains. The expression of VTCN1 is up-regulated by IL6 and IL10 and is inhibited by GM-CSF and IL4 on antigen-presenting cells (APCs). VTCN1 / B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. VTCN1 involved in promoting epithelial cell transformation.

Recombinant Biotinylated Human B7-H4, Avitag,His Tag (recommended for biopanning) (B74-H82E2) is expressed from human 293 cells (HEK293). It contains AA Phe 29 - Ala 258 (Accession # NP_078902).
Predicted N-terminus: Phe 29

Molecular Characterization
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 28 kDa. The protein migrates as 43-57 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

B74-H82E2 works best for experiments that test the binding between B7-H4 and candidate antibodies, such as biopanning and other relevant assays.

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio
Passed as determined by the HABA assay / binding ELISA.

Less than 1.0 EU per μg by the LAL method.

>90% as determined by SDS-PAGE.

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Please refer to product data sheet.

Clinical and Translational Updates

(1) "[Preparation and characterization of chicken anti-human B7-H4 IgY polyclonal antibody]"
Zhang, Fan, Zhang et al
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi (2024) 40 (3), 257-266
(2) "Docosahexaenoic acid (DHA) impairs hypoxia-induced cellular and exosomal overexpression of immune-checkpoints and immunomodulatory molecules in different subtypes of breast cancer cells"
Maralbashi, Aslan, Kahroba et al
BMC Nutr (2024) 10 (1), 41
(3) "Clinical significance of blocking novel immune checkpoint B7-H4 in urothelial carcinoma of bladder as a potential therapeutic target"
Raja, Singh, Kurra et al
Med Oncol (2024) 41 (3), 74
Showing 1-3 of 477 papers.