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Recombinant Biotinylated Human B7-H4 Protein, Avi Tag (Avitag™), His Tag, 25µg  

Recombinant Biotinylated Human B7-H4 Protein, Avi Tag (Avitag™), His Tag, 25µg

Biotinylated Human B7-H4 Protein, VTCN1 (recommended for biopanning), expressed from human 293 cells (HEK293), Avi tag (Avitag™), His Tag

Synonym
recombinant, human, biotinylated, protein, B7-H4, VTCN1, B7S1, B7h.5

More details

B74-H82E2-25

Availability: within 7 days

481,00 €

Background
V-set domain-containing T-cell activation inhibitor 1 (VTCN1) is also known as Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, B7H4, which belongs to the immunoglobulin superfamily and BTN/MOG family. VTCN1 contains two Ig-like V-type (immunoglobulin-like) domains. The expression of VTCN1 is up-regulated by IL6 and IL10 and is inhibited by GM-CSF and IL4 on antigen-presenting cells (APCs). VTCN1 / B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. VTCN1 involved in promoting epithelial cell transformation.

Source
Recombinant Biotinylated Human B7-H4, Avitag,His Tag (recommended for biopanning) (B74-H82E2) is expressed from human 293 cells (HEK293). It contains AA Phe 29 - Ala 258 (Accession # NP_078902).
Predicted N-terminus: Phe 29

Molecular Characterization
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 28 kDa. The protein migrates as 45-65 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

Application
B74-H82E2 works best for experiments that test the binding between B7-H4 and candidate antibodies, such as biopanning and other relevant assays.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio
Passed as determined by the HABA assay / binding ELISA.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "[Expression and significance of immune checkpoint B7-homolog 4 in endometrial cancer]"
Zong, Xiang, Yu et al
Zhonghua Fu Chan Ke Za Zhi (2022) 57 (12), 921-931
(2) "B7 family protein glycosylation: Promising novel targets in tumor treatment"
Xiao, Guan, Xiang et al
Front Immunol (2022) 13, 1088560
(3) "Effect of B7-H4 downregulation induced by Toxoplasma gondii infection on dysfunction of decidual macrophages contributes to adverse pregnancy outcomes"
Cui, Wang, Ren et al
Parasit Vectors (2022) 15 (1), 464
Showing 1-3 of 440 papers.